Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nasim, Zeeshan | - |
dc.contributor.author | Fahim, Muhammad | - |
dc.contributor.author | Gawarecka, Katarzyna | - |
dc.contributor.author | Susila, Hendry | - |
dc.contributor.author | Jin, Suhyun | - |
dc.contributor.author | Youn, Geummin | - |
dc.contributor.author | Ahn, Ji Hoon | - |
dc.date.accessioned | 2021-08-30T09:39:56Z | - |
dc.date.available | 2021-08-30T09:39:56Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/51953 | - |
dc.description.abstract | Nonsense-mediated mRNA decay (NMD) removes aberrant transcripts to avoid the accumulation of truncated proteins. NMD regulates nucleotide-binding, leucine-rich repeat (NLR) genes to prevent autoimmunity; however, the function of a large number of NLRs still remains poorly understood. Here, we show that three NLR genes (AT1G72910, AT1G72940, and ADR1-LIKE 2) are important for NMD-mediated regulation of defense signaling at lower temperatures. At 16 degrees C, the NMD-compromised up-frameshift protein1 (upf1) upf3 mutants showed growth arrest that can be rescued by the artificial miRNA-mediated knockdown of the three NLR genes. mRNA levels of these NLRs are induced by Pseudomonas syringae inoculation and exogenous SA treatment. Mutations in AT1G72910, AT1G72940, and ADR1-LIKE 2 genes resulted in increased susceptibility to Pseudomonas syringae, whereas their overexpression resulted in severely stunted growth, which was dependent on basal disease resistance genes. The NMD-deficient upf1 upf3 mutants accumulated higher levels of NMD signature-containing transcripts from these NLR genes at 16 degrees C. Furthermore, mRNA degradation kinetics showed that these NMD signature-containing transcripts were more stable in upf1 upf3 mutants. Based on these findings, we propose that AT1G72910, AT1G72940, and ADR1-LIKE 2 are directly regulated by NMD in a temperature-dependent manner and play an important role in modulating plant immunity at lower temperatures. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | SALICYLIC-ACID | - |
dc.subject | DEFENSE RESPONSES | - |
dc.subject | TRIGGERED IMMUNITY | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | GENE | - |
dc.subject | RESISTANCE | - |
dc.subject | GROWTH | - |
dc.subject | LIGHT | - |
dc.subject | DEGRADATION | - |
dc.subject | EXPRESSION | - |
dc.title | Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Ji Hoon | - |
dc.identifier.doi | 10.3390/ijms21217986 | - |
dc.identifier.scopusid | 2-s2.0-85094948650 | - |
dc.identifier.wosid | 000589129500001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.21 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 21 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | SALICYLIC-ACID | - |
dc.subject.keywordPlus | DEFENSE RESPONSES | - |
dc.subject.keywordPlus | TRIGGERED IMMUNITY | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | NMD | - |
dc.subject.keywordAuthor | Arabidopsis | - |
dc.subject.keywordAuthor | immunity | - |
dc.subject.keywordAuthor | NLRs | - |
dc.subject.keywordAuthor | salicylic acid | - |
dc.subject.keywordAuthor | temperature | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.