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Cited 5 time in webofscience Cited 5 time in scopus
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Independent Tuning of Nano-Ligand Frequency and Sequences Regulates the Adhesion and Differentiation of Stem Cells

Authors
Min, SunhongJeon, Yoo SangJung, Hee JoonKhatua, ChandraLi, NaBae, GunhyuChoi, HyojunHong, HyunsikShin, Jeong EunKo, Min JunKo, Han SeokJun, IndongFu, Hong EnKim, Seung HyunThangam, RamarSong, Jae-JunDravid, Vinayak P.Kim, Young KeunKang, Heemin
Issue Date
10월-2020
Publisher
WILEY-V C H VERLAG GMBH
Keywords
cell adhesion; nano-ligand frequencies; nano-ligand sequences; stem cell differentiation; unlimited nanobarcode tunability
Citation
ADVANCED MATERIALS, v.32, no.40
Indexed
SCIE
SCOPUS
Journal Title
ADVANCED MATERIALS
Volume
32
Number
40
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/52542
DOI
10.1002/adma.202004300
ISSN
0935-9648
Abstract
The native extracellular matrix (ECM) can exhibit heterogeneous nano-sequences periodically displaying ligands to regulate complex cell-material interactions in vivo. Herein, an ECM-emulating heterogeneous barcoding system, including ligand-bearing Au and ligand-free Fe nano-segments, is developed to independently present tunable frequency and sequences in nano-segments of cell-adhesive RGD ligand. Specifically, similar exposed surface areas of total Fe and Au nano-segments are designed. Fe segments are used for substrate coupling of nanobarcodes and as ligand-free nano-segments and Au segments for ligand coating while maintaining both nanoscale (local) and macroscale (total) ligand density constant in all groups. Low nano-ligand frequency in the same sequences and terminally sequenced nano-ligands at the same frequency independently facilitate focal adhesion and mechanosensing of stem cells, which are collectively effective both in vitro and in vivo, thereby inducing stem cell differentiation. The Fe/RGD-Au nanobarcode implants exhibit high stability and no local and systemic toxicity in various tissues and organs in vivo. This work sheds novel insight into designing biomaterials with heterogeneous nano-ligand sequences at terminal sides and/or low frequency to facilitate cellular adhesion. Tuning the electrodeposition conditions can allow synthesis of unlimited combinations of ligand nano-sequences and frequencies, magnetic elements, and bioactive ligands to remotely regulate numerous host cells in vivo.
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공과대학 (신소재공학부)
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