Enhancing lipid productivity by modulating lipid catabolism using the CRISPR-Cas9 system inChlamydomonas
DC Field | Value | Language |
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dc.contributor.author | Nguyen, Thu Ha Thi | - |
dc.contributor.author | Park, Seunghye | - |
dc.contributor.author | Jeong, Jooyeon | - |
dc.contributor.author | Shin, Ye Sol | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Jin, EonSeon | - |
dc.date.accessioned | 2021-08-30T13:53:25Z | - |
dc.date.available | 2021-08-30T13:53:25Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 0921-8971 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/53064 | - |
dc.description.abstract | In response to the energy crisis microalgae are a promising feedstock for biofuel production. The use of metabolic engineering to improve yields of biofuel-related lipid components in microalgae, without affecting cell growth, is now recognized as a promising and more economically feasible approach to develop more sustainable energy sources. For this, we generatedChlamydomonasmutant strains using CRISPR-Cas9 technology to knockout a gene involved in fatty acid (FA) degradation. In the knockout mutant, total lipid accumulated up to 28% of dried biomass, while that of wild-type (WT) was 22%. This increase was also accompanied by a noticeable shift in FA composition with an increase up to 27.2% in the C18:1 proportion. In addition, these mutants showed comparable growth rate to the WT, indicating that inhibiting lipid catabolism through gene editing technology is a promising strategy to develop microalgal strains for biofuel production. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | FATTY-ACID-COMPOSITION | - |
dc.subject | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject | MICROALGAE | - |
dc.subject | ACYLTRANSFERASE | - |
dc.subject | ACCUMULATION | - |
dc.subject | CGI-58 | - |
dc.subject | TRIACYLGLYCEROL | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | METABOLISM | - |
dc.subject | EXTRACTION | - |
dc.title | Enhancing lipid productivity by modulating lipid catabolism using the CRISPR-Cas9 system inChlamydomonas | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1007/s10811-020-02172-7 | - |
dc.identifier.scopusid | 2-s2.0-85087572015 | - |
dc.identifier.wosid | 000545785000001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF APPLIED PHYCOLOGY, v.32, no.5, pp.2829 - 2840 | - |
dc.relation.isPartOf | JOURNAL OF APPLIED PHYCOLOGY | - |
dc.citation.title | JOURNAL OF APPLIED PHYCOLOGY | - |
dc.citation.volume | 32 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 2829 | - |
dc.citation.endPage | 2840 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.subject.keywordPlus | FATTY-ACID-COMPOSITION | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | ACYLTRANSFERASE | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | CGI-58 | - |
dc.subject.keywordPlus | TRIACYLGLYCEROL | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordAuthor | Lipid catabolism | - |
dc.subject.keywordAuthor | CRISPR-Cas9 technology | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Chlorophyta | - |
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