Enhancing lipid productivity by modulating lipid catabolism using the CRISPR-Cas9 system inChlamydomonas
- Authors
- Nguyen, Thu Ha Thi; Park, Seunghye; Jeong, Jooyeon; Shin, Ye Sol; Sim, Sang Jun; Jin, EonSeon
- Issue Date
- 10월-2020
- Publisher
- SPRINGER
- Keywords
- Lipid catabolism; CRISPR-Cas9 technology; Metabolic engineering; Chlorophyta
- Citation
- JOURNAL OF APPLIED PHYCOLOGY, v.32, no.5, pp.2829 - 2840
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF APPLIED PHYCOLOGY
- Volume
- 32
- Number
- 5
- Start Page
- 2829
- End Page
- 2840
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/53064
- DOI
- 10.1007/s10811-020-02172-7
- ISSN
- 0921-8971
- Abstract
- In response to the energy crisis microalgae are a promising feedstock for biofuel production. The use of metabolic engineering to improve yields of biofuel-related lipid components in microalgae, without affecting cell growth, is now recognized as a promising and more economically feasible approach to develop more sustainable energy sources. For this, we generatedChlamydomonasmutant strains using CRISPR-Cas9 technology to knockout a gene involved in fatty acid (FA) degradation. In the knockout mutant, total lipid accumulated up to 28% of dried biomass, while that of wild-type (WT) was 22%. This increase was also accompanied by a noticeable shift in FA composition with an increase up to 27.2% in the C18:1 proportion. In addition, these mutants showed comparable growth rate to the WT, indicating that inhibiting lipid catabolism through gene editing technology is a promising strategy to develop microalgal strains for biofuel production.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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