Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyun Jeong | - |
dc.contributor.author | Son, Jigyeong | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Woo, Han Min | - |
dc.date.accessioned | 2021-08-30T16:03:55Z | - |
dc.date.available | 2021-08-30T16:03:55Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1467-7644 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/53669 | - |
dc.description.abstract | Designing synthetic pathways for efficient CO(2)fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO(2)into acetyl-CoA-derived acetone as an example in photosyntheticSynechococcus elongatusPCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | CARBON-DIOXIDE | - |
dc.subject | PHOTOSYNTHETIC PRODUCTION | - |
dc.subject | CO2 | - |
dc.subject | PATHWAY | - |
dc.subject | CONVERSION | - |
dc.subject | 1-BUTANOL | - |
dc.subject | FIXATION | - |
dc.subject | ETHANOL | - |
dc.title | Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1111/pbi.13342 | - |
dc.identifier.scopusid | 2-s2.0-85078912389 | - |
dc.identifier.wosid | 000557374700006 | - |
dc.identifier.bibliographicCitation | PLANT BIOTECHNOLOGY JOURNAL, v.18, no.9, pp.1860 - 1868 | - |
dc.relation.isPartOf | PLANT BIOTECHNOLOGY JOURNAL | - |
dc.citation.title | PLANT BIOTECHNOLOGY JOURNAL | - |
dc.citation.volume | 18 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1860 | - |
dc.citation.endPage | 1868 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | CARBON-DIOXIDE | - |
dc.subject.keywordPlus | PHOTOSYNTHETIC PRODUCTION | - |
dc.subject.keywordPlus | CO2 | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | 1-BUTANOL | - |
dc.subject.keywordPlus | FIXATION | - |
dc.subject.keywordPlus | ETHANOL | - |
dc.subject.keywordAuthor | cyanobacteria | - |
dc.subject.keywordAuthor | synthetic pyruvate dehydrogenase bypass | - |
dc.subject.keywordAuthor | CO(2)conversion | - |
dc.subject.keywordAuthor | metabolic engineering | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.