Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria
- Authors
- Lee, Hyun Jeong; Son, Jigyeong; Sim, Sang Jun; Woo, Han Min
- Issue Date
- 9월-2020
- Publisher
- WILEY
- Keywords
- cyanobacteria; synthetic pyruvate dehydrogenase bypass; CO(2)conversion; metabolic engineering
- Citation
- PLANT BIOTECHNOLOGY JOURNAL, v.18, no.9, pp.1860 - 1868
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANT BIOTECHNOLOGY JOURNAL
- Volume
- 18
- Number
- 9
- Start Page
- 1860
- End Page
- 1868
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/53669
- DOI
- 10.1111/pbi.13342
- ISSN
- 1467-7644
- Abstract
- Designing synthetic pathways for efficient CO(2)fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO(2)into acetyl-CoA-derived acetone as an example in photosyntheticSynechococcus elongatusPCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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