Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes
DC Field | Value | Language |
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dc.contributor.author | Kim, Duck Gyun | - |
dc.contributor.author | Yoo, Seok Woo | - |
dc.contributor.author | Kim, Minsun | - |
dc.contributor.author | Ko, Ja Kyong | - |
dc.contributor.author | Um, Youngsoon | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-08-30T18:47:57Z | - |
dc.date.available | 2021-08-30T18:47:57Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/54295 | - |
dc.description.abstract | We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | SUCCINIC ACID PRODUCTION | - |
dc.subject | CARBON CATABOLITE REPRESSION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ENHANCED PRODUCTION | - |
dc.subject | CITRATE SYNTHASE | - |
dc.subject | BINDING-SITE | - |
dc.subject | PRETREATMENT | - |
dc.subject | FERMENTATION | - |
dc.subject | GLUCOSE | - |
dc.subject | ENERGY | - |
dc.title | Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.biortech.2020.123386 | - |
dc.identifier.scopusid | 2-s2.0-85083460806 | - |
dc.identifier.wosid | 000569074800013 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.309 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 309 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | SUCCINIC ACID PRODUCTION | - |
dc.subject.keywordPlus | CARBON CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | CITRATE SYNTHASE | - |
dc.subject.keywordPlus | BINDING-SITE | - |
dc.subject.keywordPlus | PRETREATMENT | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | ENERGY | - |
dc.subject.keywordAuthor | Enterobacter aerogenes | - |
dc.subject.keywordAuthor | 2,3-butanediol | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Lignocellulosic biomass | - |
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