Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes
- Authors
- Kim, Duck Gyun; Yoo, Seok Woo; Kim, Minsun; Ko, Ja Kyong; Um, Youngsoon; Oh, Min-Kyu
- Issue Date
- 8월-2020
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Enterobacter aerogenes; 2,3-butanediol; Metabolic engineering; Lignocellulosic biomass
- Citation
- BIORESOURCE TECHNOLOGY, v.309
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 309
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54295
- DOI
- 10.1016/j.biortech.2020.123386
- ISSN
- 0960-8524
- Abstract
- We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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