Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent
- Authors
- Lee, Inseon; Cheon, Hong Jae; Adhikari, Manab Deb; Tai Duc Tran; Yeon, Kyung-Min; Kim, Moon Il; Kim, Jungbae
- Issue Date
- 15-7월-2020
- Publisher
- ELSEVIER
- Keywords
- Enzyme-inorganic hybrid nanoflowers; Glucose oxidase; Antibacterial activity
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.155, pp.1520 - 1531
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 155
- Start Page
- 1520
- End Page
- 1531
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54389
- DOI
- 10.1016/j.ijbiomac.2019.11.129
- ISSN
- 0141-8130
- Abstract
- Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH2-MNPs), called 'MNP-GOx NFs', are developed. Positively-charged NH2-MNPs and negatively- charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 10(6)/mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination. (C) 2019 Elsevier B.V. All rights reserved.
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