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Characterization of Extended-Spectrum beta-Lactamase-Producing Escherichia coli Isolated from Fresh Produce and Agricultural Environments in Korea

Authors
Cha, Min-HyeokRyu, Jae-GeeChi, Young-MinWoo, Gun-Jo
Issue Date
7월-2020
Publisher
INT ASSOC FOOD PROTECTION
Keywords
Antimicrobial resistance; CTX-M-type genes; Escherichia coli; Extended-spectrum beta-lactamase; Farm environment; Fresh produce
Citation
JOURNAL OF FOOD PROTECTION, v.83, no.7, pp.1115 - 1124
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF FOOD PROTECTION
Volume
83
Number
7
Start Page
1115
End Page
1124
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/54844
DOI
10.4315/JFP-19-483
ISSN
0362-028X
Abstract
This study was conducted to characterize Escherichia coli strains and evaluate the spread of antimicrobial resistance among these strains from fresh produce and farm environments in Korea. We then conducted phenotypic and genetic studies on antimicrobial-resistant isolates. We determined the genetic epidemiological characteristics of isolates that produced extended-spectrum beta-lactamase (ESBL) and confirmed plasmid transfer in isolates that carried bla(CTX-M)-type genes. E. coli strains were isolated from 8 samples of fresh produce and 152 samples from the farm environment collected from May 2014 to June 2016. Cephalosporin resistance was the most prevalent (61.8%) type of resistance among the isolates. Five ESBL-producing strains with high genetic homology with E. coli of human or livestock origin were identified. Lateral transfer of plasmids harboring bla(CTX-M)-type genes to transconjugants was successful. Two isolates from Chinese cabbage and from water samples collected from a nearby stream harbored the ISEcp1-bla(CTX-M-55)-orf477 operon and were confirmed as sequence type 1196 and the same type of plasmid replicon, suggesting that cross-contamination was highly likely. A high-risk clone of sequence type 69 (clonal complex 69) isolates was also recovered from the farm environment. This study provides genetic evidence that antimicrobial resistance factors in E. coli from farm environments originate in the clinic or in livestock, highlighting the fact that good agricultural practices in farming are important to inhibit the spread of antimicrobial resistance to bacteria on fresh produce.
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생명과학대학 (생명공학부)
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