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An optical detection module-based biosensor using fortified bacterial beads for soil toxicity assessment

Authors
Bae, Jin WooSeo, Ho BinBelkin, ShimshonGu, Man Bock
Issue Date
5월-2020
Publisher
SPRINGER HEIDELBERG
Keywords
Recombinant bioluminescent bacteria; PD coating; Soil toxicity biosensor module; BTEX; Optical sensors; Biosensors
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.412, no.14, pp.3373 - 3381
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume
412
Number
14
Start Page
3373
End Page
3381
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/56233
DOI
10.1007/s00216-020-02469-z
ISSN
1618-2642
Abstract
An optical biosensor module for soil contamination assessment is presented, employing bioluminescent bacterial bioreporters encapsulated in poly-dopamine (PD)-coated alginate microbeads. The PD-coated beads displayed improved mechanical strength and stability, but somewhat delayed responses to the inducing toxicant. Using toluene as a model soil contaminant, two bioluminescent reporter strains were employed for its detection in the ambient light-blocking, temperature-controlled biosensor module. Bioluminescence of strain TV1061 (harboring an inducible grpE::luxCDABE fusion) increased and that of strain GC2 (harboring a constitutive lac::luxCDABE fusion) decreased in the presence of increasing toluene concentrations. In the former case, a maximal effect was observed in the presence of 1% toluene. This simple optical detection biosensor module may potentially be utilized for monitoring soil contamination from areas suspected of chemical pollution such petrochemical industrial zones or petrol stations.
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