Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry
- Authors
- Han, Yoontak; Lee, Eun-Jin
- Issue Date
- 4월-2020
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Keywords
- Salmonella Typhimurium; Type II flagella
- Citation
- JOURNAL OF MICROBIOLOGY, v.58, no.4, pp.245 - 251
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY
- Volume
- 58
- Number
- 4
- Start Page
- 245
- End Page
- 251
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/56830
- DOI
- 10.1007/s12275-020-9297-y
- ISSN
- 1225-8873
- Abstract
- The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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