Synergistic staphylocidal interaction of benzoic acid derivatives (benzoic acid, 4-hydroxybenzoic acid and beta-resorcylic acid) and capric acid: mechanism and verification study using artificial skin
- Authors
- Kim, H. W.; Seok, Y. S.; Rhee, M. S.
- Issue Date
- 3월-2020
- Publisher
- OXFORD UNIV PRESS
- Citation
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, v.75, no.3, pp.571 - 575
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
- Volume
- 75
- Number
- 3
- Start Page
- 571
- End Page
- 575
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/57488
- DOI
- 10.1093/jac/dkz494
- ISSN
- 0305-7453
- Abstract
- Objectives: The present study was designed to investigate a synergistic staphylocidal interaction of antimicrobials. Methods: The widely used preservative benzoic acid (BzA) and its derivatives [4-hydroxybenzoic acid (HA) and beta-resorcylic acid (beta-RA)] combined with capric acid (CPA) were investigated. Results: beta-RA was identified as the most effective antimicrobial exhibiting synergistic action with CPA against both Staphylococcus aureus and MRSA. For example, a complete reduction of bacteria (>7.3 log reduction) was obtained within 5 min after treatment with 5.0 mM beta-RA (0.079%) plus 0.20 mM CPA (0.004%), while treatment with each material individually showed low bactericidal effects (<1.5 log reduction). Flow cytometry analysis identified membrane disruption related to the synergistic mechanisms, including the following: (i) membrane disruption by CPA (69.2% of cells were damaged by 0.20 mM CPA treatment); (ii) antimicrobial entry through the damaged membrane; and (iii) cytoplasmic ion imbalance resulting in cell death. We verified that the synergistic combination was also effective against MRSA on artificial skin (99.989% elimination after 5 min). Conclusions: We used only consumer-preferred natural-borne antimicrobials and a very small amount of material was needed based on the synergistic effects. Therefore, these antimicrobials can be widely used as alternative anti-MRSA compounds in healthcare products, cosmetics, pharmaceutical products, foods and for environmental hygiene.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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