Development of a Multiplex RT-qPCR for the Detection of Different Clades of Avian Influenza in Poultry
- Authors
- Tran Bac Le; Kim, Hye Kwon; Na, Woonsung; Van Phan Le; Song, Min-Suk; Song, Daesub; Jeong, Dae Gwin; Yoon, Sun-Woo
- Issue Date
- 1월-2020
- Publisher
- MDPI
- Keywords
- HPAI; H5Nx; Clade; simultaneous detection; field samples
- Citation
- VIRUSES-BASEL, v.12, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- VIRUSES-BASEL
- Volume
- 12
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/58572
- DOI
- 10.3390/v12010100
- ISSN
- 1999-4915
- Abstract
- Since the initial detection of H5N1, a highly pathogenic avian influenza (HPAI) virus, in 1996 in China, numerous HPAI H5 lineages have been classified, and they continue to pose a threat to animal and human health. In this study, we developed a novel primer/probe set that can be employed to simultaneously detect pan-H5 HPAI and two clades, 2.3.2.1 and 2.3.4.4, of H5Nx viruses using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The sensitivity and specificity of these primer sets and probes were confirmed with a number of different subtypes of influenza virus and the H5-HA gene plasmid DNA. In particular, the multiplex RT-qPCR assay was successfully applied to the simultaneous detection of H5 HPAI and different virus clades in clinical field samples from a poultry farm. Therefore, this multiplex assay and a novel detection primer set and probes will be useful for the laboratory diagnosis and epidemiological field studies of different circulating H5 HPAI virus clades in poultry and migratory wild birds.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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