Muricauda ochracea sp. nov., isolated from a tidal flat in the Republic of Korea

Abstract

A yellowish-brown-coloured bacterium, designated strain JGD-17(T), was isolated from a tidal flat of Janggu-do. Garorim bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and long-rodshaped. Growth was observed at 20-45 degrees C (optimum, 25-30 degrees C), at pH 6.0-10.0 (9.0) and with 1-5% (w/v) NaCl (1-3%). Results of 16S rRNA gene sequence analysis indicated that strain JGD-17(T) was closely related to Muricauda nanhaiensis SM1704(T) (96.1%), Muricauda olearia CL-SS4(T) (95.0%), Muricauda beolgyonensis BB-My12(T) (94.9%), Muricauda marina H19-56(T) (94.7%) and Muricauda indica 3PC125-7(T) (94.5%). The ranges of values for the average nucleotide identity and digital DNA-DNA hybridization analyses with related strains were 71.3-74.1%and 16.9-18.2%. The genomic DNA G+C content was 41.1 mol%. Phylogenetic analysis using the neighbour-joining method showed that strain JGD-17(T) formed a Glade with Muricauda nanhaiensis SM1704(T), Muricauda lutaonensis CC-HSB-11(T), Muricauda lutea CSWOC and Muricauda pacifica SM027(T). The major fatty acids were iso-C-15.0 (26.9%), iso-C-15:1 G (19.5%) and iso-C-17:0 3-OH (12.7%). The predominant respiratory quinone was menaquinone-6. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain JGD-17(T) represents a novel species within the genus Muricauda, for which the name Muricauda ochracea sp. nov. is proposed. The type strain is JGD-17(T) (=KCTC 72732(T) =KACC 21486(T)=JCM 33817(T)).