Ultrafast Chemical Exchange Dynamics of Hydrogen Bonds Observed via Isonitrile Infrared Sensors: Implications for Biomolecular Studies
- Authors
- Kubel, Joachim; Lee, Giseong; Ooi, Saik Ann; Westenhoff, Sebastian; Han, Hogyu; Cho, Minhaeng; Maj, Michal
- Issue Date
- 19-12월-2019
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF PHYSICAL CHEMISTRY LETTERS, v.10, no.24, pp.7878 - 7883
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF PHYSICAL CHEMISTRY LETTERS
- Volume
- 10
- Number
- 24
- Start Page
- 7878
- End Page
- 7883
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/60897
- DOI
- 10.1021/acs.jpclett.9b03144
- ISSN
- 1948-7185
- Abstract
- Local probes are indispensable to study protein structure and dynamics with site-specificity. The isonitrile functional group is a highly sensitive and H-bonding interaction-specific probe. Isonitriles exhibit large spectral shifts and transition dipole moment changes upon H-bonding while being weakly affected by solvent polarity. These unique properties allow a clear separation of distinct subpopulations of interacting species and an elucidation of their ultrafast dynamics with two-dimensional infrared (2D-IR) spectroscopy. Here, we apply 2D-IR to quantify the picosecond chemical exchange dynamics of solute solvent complexes forming between isonitrile-derivatized alanine and fluorinated ethanol, where the degree of fluorination controls their H-bond-donating ability. We show that the molecules undergo faster exchange in the presence of more acidic H-bond donors, indicating that the exchange process is primarily dependent on the nature of solvent-solvent interactions. We foresee isonitrile as a highly promising probe for studying of H-bonds dynamics in the active site of enzymes.
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