The Pseudomonas aeruginosa HSP70-like protein DnaK induces IL-1 beta expression via TLR4-dependent activation of the NF-kappa B and JNK signaling pathways
- Authors
- Lee, Jung-Hoon; Jeon, Jisu; Bai, Fang; Jin, Shouguang; Wu, Weihui; Ha, Un-Hwan
- Issue Date
- 12월-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- DnaK; IL-1 beta; JNK; NF-kappa B; Pseudomonas aeruginosa; TLR4
- Citation
- COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v.67
- Indexed
- SCIE
SCOPUS
- Journal Title
- COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
- Volume
- 67
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/61306
- DOI
- 10.1016/j.cimid.2019.101373
- ISSN
- 0147-9571
- Abstract
- IL-1 beta expression is increased in response to P. aeruginosa infection, but the responsible proteins have not been clearly elucidated. Here, we demonstrate for the first time that IL-1 beta expression is induced in response to the heat shock protein 70-like protein DnaK. Treatment with recombinant DnaK (rDnaK) increased IL-1 beta expression in a dose- and time-dependent manner, and the release of mature IL-1 beta in response to rDnaK was detected to an extent similar to that stimulated by the well-known agonists, lipopolysaccharide and nigericin. rDnaK-mediated IL-1 beta expression was driven by the NF-kappa B signaling pathway. In addition, expression was controlled by the JNK signaling pathway, although these two signaling cascades act independently upon rDnaK stimulation. Finally, rDnaK-induced IL-1 beta expression was initiated via the action of TLR4. Taken together, the data reveal that P. aeruginosa-derived DnaK induces expression of IL-1 beta via TLR4-dependent activation of the NF-kappa B and JNK signaling pathways.
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Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
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