Pathogenesis of Enterohemorrhagic Escherichia coli O157:H7 is mediated by the cytochrome P450 family in Caenorhabditis elegans animal model
DC Field | Value | Language |
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dc.contributor.author | Ryu, S. | - |
dc.contributor.author | Oh, S. | - |
dc.contributor.author | Park, M. R. | - |
dc.contributor.author | Lee, W. J. | - |
dc.contributor.author | Yan, B. | - |
dc.contributor.author | Choi, H. J. | - |
dc.contributor.author | Oh, M. H. | - |
dc.contributor.author | Oh, N. S. | - |
dc.contributor.author | Song, M. H. | - |
dc.contributor.author | Kim, Y. | - |
dc.date.accessioned | 2021-09-01T07:18:21Z | - |
dc.date.available | 2021-09-01T07:18:21Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 0956-7135 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62963 | - |
dc.description.abstract | Foodborne pathogens, including enterohemorrhagic Escherichia coli. (EHEC) O157:H7, may enter from the farm environment and foods via several different vectors and influence human health. Here, we employed Caenorhabditis elegans as a host model system and compared specific host responses during EHEC O157:H7 infection using whole-transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157:H7, we employed quantitative real-time polymerase chain reaction (qRT-PCR), transgenic worms, and RNAi. Whole-transcriptome analysis revealed that genes encoding the cytochrome P450 (CYP450) family were induced more than 10-fold during EHEC O157:H7 infection in C. elegans host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infection compared with wild-type N2 worms. Consistent with susceptibility tests, qRT-PCR results indicated that CYP450 loss-of-function mutations significantly affected the transcriptional induction of antimicrobial peptide genes, such as clec-60. Together, our results provide critical insights into host strategies for avoiding EHEC O157:H7 pathogenesis in the gastrointestinal tract via the cytochrome P450 family and highlights potential molecular targets for preventing the virulence of EHEC O157:H7 in foods. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | HOST | - |
dc.subject | INFECTION | - |
dc.subject | VIRULENCE | - |
dc.subject | BACTERIA | - |
dc.subject | DEFENSE | - |
dc.subject | H7 | - |
dc.title | Pathogenesis of Enterohemorrhagic Escherichia coli O157:H7 is mediated by the cytochrome P450 family in Caenorhabditis elegans animal model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, N. S. | - |
dc.identifier.doi | 10.1016/j.foodcont.2019.03.036 | - |
dc.identifier.scopusid | 2-s2.0-85064862356 | - |
dc.identifier.wosid | 000467664100022 | - |
dc.identifier.bibliographicCitation | FOOD CONTROL, v.103, pp.182 - 185 | - |
dc.relation.isPartOf | FOOD CONTROL | - |
dc.citation.title | FOOD CONTROL | - |
dc.citation.volume | 103 | - |
dc.citation.startPage | 182 | - |
dc.citation.endPage | 185 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | HOST | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.subject.keywordPlus | H7 | - |
dc.subject.keywordAuthor | EHEC O157:H7 | - |
dc.subject.keywordAuthor | C. elegans | - |
dc.subject.keywordAuthor | Cytochrome P450 family | - |
dc.subject.keywordAuthor | Immune response | - |
dc.subject.keywordAuthor | Food safety | - |
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