Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG-DNA-Liposome Complex
- Authors
- Park, Byoung Kwon; Lee, Su In; Bae, Joon-Yong; Park, Man-Seong; Lee, Younghee; Kwon, Hyung-Joo
- Issue Date
- Sep-2019
- Publisher
- SPRINGER
- Keywords
- B cell epitope; Monoclonal antibody; MERS-CoV; M protein
- Citation
- INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, v.25, no.3, pp.819 - 826
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
- Volume
- 25
- Number
- 3
- Start Page
- 819
- End Page
- 826
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/62991
- DOI
- 10.1007/s10989-018-9731-8
- ISSN
- 1573-3149
- Abstract
- The Middle East respiratory syndrome-related coronavirus (MERS-CoV) contains four major structural proteins, the spike glycoprotein, nucleocapsid phosphoprotein, membrane (M) glycoprotein and small envelope glycoprotein. The M protein of MERS-CoV has a role in the morphogenesis or assembly of the virus and inhibits type I interferon expression in infected cells. Here, we produced a monoclonal antibody specific against the M protein of MERS-CoV by injecting BALB/c mice with a complex containing the epitope peptide and CpG-DNA encapsulated with a phosphatidyl-beta-oleoyl-gamma-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS). The monoclonal antibody was reactive to the epitope peptide of the M protein of MERS-CoV which was confirmed by western blotting and immunoprecipitations. Indirect immunofluorescence assay and confocal image analysis showed that the monoclonal antibody binds specifically to the M protein of MERS-CoV in the virus-infected cells. Further studies using this monoclonal antibody may provide important information on the function of the M protein and its future application in diagnostics.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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