Effect of cross-linking on the dimensional stability and biocompatibility Check tor of a tailored 3D-bioprinted gelatin scaffold
- Authors
- Choi, Dong Jin; Kho, YoungJae; Park, Sang Jun; Kim, Young-Jin; Chung, Seok; Kim, Chun-Ho
- Issue Date
- 15-8월-2019
- Publisher
- ELSEVIER
- Keywords
- 3D bioprinting; Gelatin hydrogel; Dried heat treatment; Cross-linking; Tissue engineering
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.135, pp.659 - 667
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 135
- Start Page
- 659
- End Page
- 667
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/63519
- DOI
- 10.1016/j.ijbiomac.2019.05.207
- ISSN
- 0141-8130
- Abstract
- Biocompatible and biodegradable gelatin is a good candidate bioink for use in 3D bioprinting technologies, but viscous gelatin solution has a low printability. In order to improve the poor printability of gelatin, we optimized the rheological properties of gelatin solution. 3D gelatin scaffolds were then cross-linked using physical or chemical methods to maintain the 3D structure. The physicochemical and biological differences between the two types of cross-linked gelatin scaffolds were studied. Scanning electron microscopy images revealed that the morphologies of the resulting cross-linked 3D scaffolds maintained their structural stabilities. The physically cross-linked 3D scaffolds maintained their surface sizes without a significant decrease (less than a 3% reduction in the surface size was observed) after cross-linking. To evaluate the differences in cell affinity by two types of cross-linking method, human dermal fibroblasts cultured on the cross-linked 3D scaffolds. After 14 days of culturing, DNA assays showed that the cell proliferation rate of the physically cross-linked 3D scaffold was 44% higher than that of the chemically cross-linked 3D scaffold. In conclusion, the optimized physically cross-linked 3D scaffold retained its surface size without significant decreases after cross-linking, as required by 3D-printed patient-specific tissue engineered customized scaffolds, despite the use of water-soluble gelatin hydrogels. (C) 2019 Elsevier B.V. All rights reserved.
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