A chemiluminescence resonance energy transfer strategy and its application for detection of platinum ions and cisplatin
- Authors
- Cai, Sheng; Zhou, Ying; Ye, Jiawei; Chen, Ruizhe; Sun, Lianli; Lu, Jianzhong; Jung, Cheulhee; Zeng, Su
- Issue Date
- 7월-2019
- Publisher
- SPRINGER WIEN
- Keywords
- CRET; Streptavidin aptamer; Platinum-oligonucleotide coordination; Platinum aptameric platform; Platinum detection
- Citation
- MICROCHIMICA ACTA, v.186, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 186
- Number
- 7
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/64239
- DOI
- 10.1007/s00604-019-3509-3
- ISSN
- 0026-3672
- Abstract
- A novel chemiluminescence resonance energy transfer (CRET) system was developed and combined with a structure-switching aptamer for the highly sensitive detection of platinum. Platinum was chosen as a model analyte to demonstrate the generality of the new CRET system. This aptameric platform consisted of a streptavidin labeled aptamer against platinum and a streptavidin-coated magnetic bead for the selective separation of platinum-bound aptamer. The platinum-aptamer probe contained several guanine (G) bases bound to the 3,4,5-trimethoxyphenyl-glyoxal (TMPG) donor group at the 5 end, a fluorescent acceptor (6-carboxy-2,4,7,7-tetrachlorofluorescein, TET) at the 3 end, and a streptavidin aptamer sequence in which several base pairs were replaced by the G-G mismatch to induce the platinum-oligonucleotide coordination. The chemiluminescence (CL) generated by TMPG/G bases is transferred to the acceptor (TET). In the presence of platinum, the platinum-aptamer probe was folded such that the G bases at the 5 end and TET at the 3 were in close proximity. The complex was separated using streptavidin-coated magnetic beads by the addition of TMPG to form the TMPG/G bases complex. The ultraweak CL from the TMPG/G bases was strongly enhanced by TET. This novel CRET-based method can be easily performed with high limit of detection (50ngmL(-1)) and selectivity over other metal ions. This technique provides a novel method for simple, fast, and convenient point-of-care diagnostics for monitoring proteins and metal ions.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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