Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay
DC Field | Value | Language |
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dc.contributor.author | Kim, Inhong | - |
dc.contributor.author | Jung, Ji-Eun | - |
dc.contributor.author | Lee, Woojin | - |
dc.contributor.author | Park, Seongho | - |
dc.contributor.author | Kim, Heedae | - |
dc.contributor.author | Jho, Young-Dahl | - |
dc.contributor.author | Woo, Han Young | - |
dc.contributor.author | Kyhm, Kwangseuk | - |
dc.date.accessioned | 2021-09-01T13:30:00Z | - |
dc.date.available | 2021-09-01T13:30:00Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64662 | - |
dc.description.abstract | We recently implemented highly sensitive detection systems for photo-sensitizing potassium ions (K+) based on two-step Forster resonance energy transfer (FRET). As a successive study for quantitative understanding of energy transfer processes in terms of the exciton population, we investigated the fluorescence decay dynamics in conjugated polymers and an aptamer-based 6-carboxyfluorescein (6-FAM)/6-carboxytetramethylrhodamine (TAMRA) complex. In the presence of K+ ions, the Guanine-rich aptamer enabled efficient two-step resonance energy transfer from conjugated polymers to dyed pairs of 6-FAM and TAMRA through the G-quadruplex phase. Although the fluorescence decay time of TAMRA barely changed, the fluorescence intensity was significantly increased. We also found that 6-FAM showed a decreased exciton population due the compensation of energy transfer to TAMRA by FRET from conjugated polymers, but a fluorescence quenching also occurred concomitantly. Consequently, the fluorescence intensity of TAMRA showed a 4-fold enhancement, where the initial transfer efficiency (similar to 300%) rapidly saturated within similar to 0.5 ns and the plateau of transfer efficiency (similar to 230%) remained afterward. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | G-QUADRUPLEX | - |
dc.subject | FRET | - |
dc.subject | SINGLE | - |
dc.subject | POLYELECTROLYTE | - |
dc.subject | EFFICIENCY | - |
dc.subject | ACCEPTORS | - |
dc.title | Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Woo, Han Young | - |
dc.identifier.doi | 10.3390/polym11071206 | - |
dc.identifier.scopusid | 2-s2.0-85070391832 | - |
dc.identifier.wosid | 000480539500123 | - |
dc.identifier.bibliographicCitation | POLYMERS, v.11, no.7 | - |
dc.relation.isPartOf | POLYMERS | - |
dc.citation.title | POLYMERS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | G-QUADRUPLEX | - |
dc.subject.keywordPlus | FRET | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | POLYELECTROLYTE | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | ACCEPTORS | - |
dc.subject.keywordAuthor | FRET | - |
dc.subject.keywordAuthor | time-resolved photoluminescence | - |
dc.subject.keywordAuthor | two-step FRET | - |
dc.subject.keywordAuthor | potassium ion detection | - |
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