Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay
- Authors
- Kim, Inhong; Jung, Ji-Eun; Lee, Woojin; Park, Seongho; Kim, Heedae; Jho, Young-Dahl; Woo, Han Young; Kyhm, Kwangseuk
- Issue Date
- 7월-2019
- Publisher
- MDPI
- Keywords
- FRET; time-resolved photoluminescence; two-step FRET; potassium ion detection
- Citation
- POLYMERS, v.11, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- POLYMERS
- Volume
- 11
- Number
- 7
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/64662
- DOI
- 10.3390/polym11071206
- ISSN
- 2073-4360
- Abstract
- We recently implemented highly sensitive detection systems for photo-sensitizing potassium ions (K+) based on two-step Forster resonance energy transfer (FRET). As a successive study for quantitative understanding of energy transfer processes in terms of the exciton population, we investigated the fluorescence decay dynamics in conjugated polymers and an aptamer-based 6-carboxyfluorescein (6-FAM)/6-carboxytetramethylrhodamine (TAMRA) complex. In the presence of K+ ions, the Guanine-rich aptamer enabled efficient two-step resonance energy transfer from conjugated polymers to dyed pairs of 6-FAM and TAMRA through the G-quadruplex phase. Although the fluorescence decay time of TAMRA barely changed, the fluorescence intensity was significantly increased. We also found that 6-FAM showed a decreased exciton population due the compensation of energy transfer to TAMRA by FRET from conjugated polymers, but a fluorescence quenching also occurred concomitantly. Consequently, the fluorescence intensity of TAMRA showed a 4-fold enhancement, where the initial transfer efficiency (similar to 300%) rapidly saturated within similar to 0.5 ns and the plateau of transfer efficiency (similar to 230%) remained afterward.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.