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Inhibition of Listeria monocytogenes using biofilms of non-pathogenic soil bacteria (Streptomyces spp.) on stainless steel under desiccated condition

Authors
Kim, YoonbinKim, HoikyungBeuchat, Larry R.Ryu, Jee-Hoon
Issue Date
6월-2019
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Keywords
Streptomyces spp.; Biofilm; Listeria monocytogenes; Desiccation; Stainless steel surface
Citation
FOOD MICROBIOLOGY, v.79, pp.61 - 65
Indexed
SCIE
SCOPUS
Journal Title
FOOD MICROBIOLOGY
Volume
79
Start Page
61
End Page
65
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/64886
DOI
10.1016/j.fm.2018.11.007
ISSN
0740-0020
Abstract
Of the 1648 microbial isolates from 133 soil samples collected from 30 diverse locations in the Republic of Korea, two isolates exhibited strong antilisterial activity and ability to grow to high populations (> 8.0 log CFU/ml) in Bennett's broth. Isolates were identified as Streptomyces lactacystinicus (strain Samnamu 5-15) and Streptomyces purpureus (strain Chamnamu-sup 4-15). Both isolates formed biofilms on the surface of stainless steel coupons (SSCs) immersed in Bennett's broth within 24 h at 25 degrees C. Cells retained antilisterial activity after biofilm formation and showed significantly (P <= 0.05) enhanced resistance against dry conditions (43% relative humidity [RH]) compared to the cells not in biofilm. An initial population (ca. 3.2 log CFU/cm(2)) of Listeria monocytogenes inoculated on SSCs lacking Streptomyces biofilm decreased to 1.4 log CFU/cm(2) within 48 h at 25 degrees C and 43% RH. In contrast, L. monocytogenes (3.3 log CFU/cm(2)) inoculated on SSCs containing Streptomyces biofilm decreased to populations below the theoretical detection limit (0.5 log CFU/cm(2)) within 24 h. The results indicate that biofilms formed by the Streptomyces spp. inhibitory to L. monocytogenes showed enhanced resistance to desiccation condition (43% RH) and effectively inhibited the growth of L. monocytogenes on the surfaces of SSCs. Antilisterial biofilms developed in this study may be applicable on desiccated environmental surfaces in food related environments such as food storage, handling, and processing facilities to enhance the microbiological safety of foods.
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