Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat

Abstract

A novel cream-pigmented marine bacterium, designated strain YJ-T1-11(T), was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11(T) clustered with Gemmobacter fontiphilus JS43(T) (98.3 %) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857(T) (98.5 %), Gemmobacter aquaticus A1-9(T) (98.4 %), Gemmobacter lanyuensis Orc-4(T) (98.4 %), Gemmobacter fontiphilus JS43 T (98.3 %), Gemmobacter caeni DCA-1(T) (98.2 %), Gemmobacter nanjingensis Y12(T) (97.5%) and Gemmobacter tilapiae Ruye-53(T) (97.2%). Average nucleotide identity values between the genome sequences of strain YJ-T1-11(T) and the related type strains ranged from 77.08 to 90.48%. The predominant fatty acid of strain YJ-T1-11(T) was summed feature 8 (comprising C-18(:1)omega 7c and/or C-18(:1)omega 6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11(T) and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11(T) is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11(T) (=KCTC 62715(T)=JCM 32828(T)).