Brassinin, a phytoalexin in cruciferous vegetables, suppresses obesity-induced inflammatory responses through the Nrf2-HO-1 signaling pathway in an adipocyte-macrophage co-culture system
DC Field | Value | Language |
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dc.contributor.author | Kang, Bobin | - |
dc.contributor.author | Kim, Chae Young | - |
dc.contributor.author | Hwang, Jisu | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.contributor.author | Choi, Hyeon-Son | - |
dc.date.accessioned | 2021-09-01T15:49:14Z | - |
dc.date.available | 2021-09-01T15:49:14Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 0951-418X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/65898 | - |
dc.description.abstract | The aim of this study was to investigate the effect of brassinin (BR), a phytoalexin found in plants belonging to the Brassicaceae family, on the obesity-induced inflammatory response and its molecular mechanism in co-culture of 3T3-L1 adipocytes and RAW264.7 macrophages. BR effectively suppressed lipid accumulation by down-regulating the expression of adipogenic factors, which in turn, were regulated by early adipogenic factors such as CCAAT-enhancer-binding protein-beta and Kruppel-like factor 2. Production of inflammatory cytokines and reactive oxygen species, induced by adipocyte-conditioned medium, was significantly decreased in BR-treated cells. This effect of BR was more prominent in contact co-culture of adipocytes and macrophages with a 90% and 34% reduction in IL-6 and MCP-1 levels, respectively. BR also restored adiponectin expression, which was significantly reduced by culturing adipocytes in macrophage-conditioned medium. In the transwell system, BR increased the protein levels of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target molecule, hemoxygenase-1 (HO-1), by 55%-93% and 45%-48%, respectively, and also increased Nrf2 translocation into the nucleus. However, knockdown of Nrf2 or HO-1 in RAW264.7 cells restored this BR-mediated inhibition of IL-6 and MCP-1 production. These results indicated that BR inhibited obesity-induced inflammation via the Nrf2-HO-1 pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | HEME OXYGENASE-1 | - |
dc.subject | CELL-CYCLE | - |
dc.subject | ADIPOGENESIS | - |
dc.subject | NRF2 | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | SULFORAPHANE | - |
dc.subject | INHIBITION | - |
dc.subject | ROLES | - |
dc.title | Brassinin, a phytoalexin in cruciferous vegetables, suppresses obesity-induced inflammatory responses through the Nrf2-HO-1 signaling pathway in an adipocyte-macrophage co-culture system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1002/ptr.6333 | - |
dc.identifier.scopusid | 2-s2.0-85062784153 | - |
dc.identifier.wosid | 000469402900016 | - |
dc.identifier.bibliographicCitation | PHYTOTHERAPY RESEARCH, v.33, no.5, pp.1426 - 1437 | - |
dc.relation.isPartOf | PHYTOTHERAPY RESEARCH | - |
dc.citation.title | PHYTOTHERAPY RESEARCH | - |
dc.citation.volume | 33 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1426 | - |
dc.citation.endPage | 1437 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | CELL-CYCLE | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | SULFORAPHANE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordAuthor | brassinin | - |
dc.subject.keywordAuthor | co-culture | - |
dc.subject.keywordAuthor | nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-hemoxygenase-1 (HO-1) signaling pathway | - |
dc.subject.keywordAuthor | obesity-induced inflammation | - |
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