Angiotensin inhibition in the developing kidney; tubulointerstitial effect
- Authors
- Yoo, Kee Hwan; Yim, Hyung Eun; Bae, Eun Soo; Hong, Young Sook
- Issue Date
- 4월-2019
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- PEDIATRIC RESEARCH, v.85, no.5, pp.724 - 730
- Indexed
- SCIE
SCOPUS
- Journal Title
- PEDIATRIC RESEARCH
- Volume
- 85
- Number
- 5
- Start Page
- 724
- End Page
- 730
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/66485
- DOI
- 10.1038/s41390-019-0288-9
- ISSN
- 0031-3998
- Abstract
- BACKGROUND: Renin-angiotensin system (RAS) blockade during nephrogenesis causes a broad range of renal mal-development. Here, we hypothesized that disruption of renal lymphangiogenesis may contribute to tubulointerstitial alterations after RAS blockade during kidney maturation. METHODS: Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle for 7 days after birth. Lymphangiogenesis was assessed via immunostaining and/or immunoblots for vascular endothelial growth factor (VEGF)-C, VEGF receptor (VEGFR)-3, Podoplanin, and Ki-67. The intrarenal expression of fibroblast growth factor (FGF)-1, FGF-2, FGF receptor (R)-1, alpha-smooth muscle actin (alpha-SMA), and fibroblast-specific protein (FSP)-1 was also determined. Sirius Red staining was performed to evaluate interstitial collagen deposition. RESULTS: On postnatal day 8, renal lymphangiogenesis was disrupted by neonatal enalapril treatment. The expression of podoplanin and Ki-67 decreased in enalapril-treated kidneys. While the expression of VEGF-C was decreased, the levels of VEGFR-3 receptor increased following enalapril treatment. Enalapril treatment also reduced the renal expression of FGF-1, FGF-2, and FGFR-1. Enalapril-treated kidneys exhibited profibrogenic properties with increased expression of alpha-SMA and FSP-1 and enhanced deposition of interstitial collagen. CONCLUSION: Enalapril treatment during postnatal renal maturation can disrupt renal lymphangiogenesis along with tubulointerstitial changes, which may result in a pro-fibrotic environment in the developing rat kidney.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.