Linseed polysaccharides based nanoparticles for controlled delivery of docetaxel: Design, in vitro drug release and cellular uptake

Abstract

Linseed polysaccharides (LSP) were used to develop a nanoparticle carrier system for the effective delivery of docetaxel (DTX). DTX was loaded in LSP core and further protected by pluronic F-68 shell. Formation, morphology and size distribution of DTX loaded LSP-pluronic nanoparticles (DLP-NPs) were characterized by DLS, FESEM, and PXRD. Spherical shaped DLP-NPs were observed using FESEM image having diameter to be 155 +/- 44 nm depending on the drug loading (DL). Encapsulation efficiency (EE) and DL was found in the range from 98 to 78.33% and 0.98-2.35%, respectively. In vitro drug release studies showed prolong (more than 96 h) and sustain release of DTX from DLP-NPs. The cytotoxicity and cellular uptake study indicated the minimum toxicity of LSP and formulation was found chemotherapeutically effective. Results of the current study demonstrated a potential application of naturally occurring biopolymers in building novel hydrophilic nanoparticle formulation for delivering anticancer therapeutic agents.