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Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1

Authors
Kim, Kem OkLee, DahaeNguyen Tuan HiepSong, Ji HoonLee, Hae-JeungLee, DonghoKang, Ki Sung
Issue Date
1-1월-2019
Publisher
MDPI
Keywords
contrast agent; iodixanol; cytotoxicity; MAPKs; caspase-3; kidney injury molecule-1
Citation
MOLECULES, v.24, no.1
Indexed
SCIE
SCOPUS
Journal Title
MOLECULES
Volume
24
Number
1
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/68366
DOI
10.3390/molecules24010195
ISSN
1420-3049
Abstract
We investigated whether 14 phenolic compounds isolated from Artemisia argyi could prevent the apoptotic damage caused by iodixanol, an iodinated contrast agent, on LLC-PK1 cells. Iodixanol was used to induce cytotoxicity in LLC-PK1 cells. Apoptotic cell death was observed as the fluorescence intensity emitted by annexin V and Hoechst 33342 stains. Western blotting was used to detect specific proteins. Seven phenolic compounds protected against iodixanol-induced LLC-PK1 cell death in a concentration-dependent manner. Among them, methyl caffeate exerted the strongest protective effect, and co-treatment with 50 and 100 M methyl caffeate decreased intracellular reactive oxygen species elevated by 25 mg/mL iodixanol. In addition, the treatment of LLC-PK1 cells with iodixanol resulted in an increase in apoptotic cell death, which decreased by co-treatment with methyl caffeate. Iodixanol caused a cytotoxicity-related increase in the phosphorylation of extracellular-signal-regulated kinase, c-Jun N-terminal kinase, and P38; and a similar increase in the expression levels of kidney injury molecule-1 and cleaved caspase-3. However, the up-regulation of these proteins was reversed by co-treatment with methyl caffeate. These findings suggest that phenolic compounds isolated from A. argyi play an important role in protecting kidney epithelium cells against apoptotic damage caused by iodixanol.
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