Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
DC Field | Value | Language |
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dc.contributor.author | Kim, Hee Taek | - |
dc.contributor.author | Khang, Tae Uk | - |
dc.contributor.author | Baritugo, Kei-Anne | - |
dc.contributor.author | Hyun, Sung Min | - |
dc.contributor.author | Kang, Kyoung Hee | - |
dc.contributor.author | Jung, Sol Hee | - |
dc.contributor.author | Song, Bong Keun | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.contributor.author | Kim, Gi Bae | - |
dc.contributor.author | Kim, Hyun Uk | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.contributor.author | Park, Si Jae | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.date.accessioned | 2021-09-01T21:47:06Z | - |
dc.date.available | 2021-09-01T21:47:06Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/68401 | - |
dc.description.abstract | Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His(6)-tag to improve the production of glutaric acid. It was found that use of N-terminal His(6)-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His(6)-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | BIO-BASED PRODUCTION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | PSEUDOMONAS-PUTIDA | - |
dc.subject | LYSINE CATABOLISM | - |
dc.subject | EXPRESSION | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | 5-AMINOVALERATE | - |
dc.subject | DEHYDROGENASE | - |
dc.subject | BIOTECHNOLOGY | - |
dc.subject | PATHWAY | - |
dc.title | Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.ymben.2018.08.007 | - |
dc.identifier.scopusid | 2-s2.0-85057062236 | - |
dc.identifier.wosid | 000456420200011 | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.51, pp.99 - 109 | - |
dc.relation.isPartOf | METABOLIC ENGINEERING | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 51 | - |
dc.citation.startPage | 99 | - |
dc.citation.endPage | 109 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | BIO-BASED PRODUCTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PSEUDOMONAS-PUTIDA | - |
dc.subject.keywordPlus | LYSINE CATABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | 5-AMINOVALERATE | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | BIOTECHNOLOGY | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Glutaric acid | - |
dc.subject.keywordAuthor | L-Lysine | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | davTDBA | - |
dc.subject.keywordAuthor | Codon optimization | - |
dc.subject.keywordAuthor | His(6)-tag | - |
dc.subject.keywordAuthor | Fed-batch fermentation | - |
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