Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
- Authors
- Kim, Hee Taek; Khang, Tae Uk; Baritugo, Kei-Anne; Hyun, Sung Min; Kang, Kyoung Hee; Jung, Sol Hee; Song, Bong Keun; Park, Kyungmoon; Oh, Min-Kyu; Kim, Gi Bae; Kim, Hyun Uk; Lee, Sang Yup; Park, Si Jae; Joo, Jeong Chan
- Issue Date
- 1월-2019
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Glutaric acid; L-Lysine; Corynebacterium glutamicum; davTDBA; Codon optimization; His(6)-tag; Fed-batch fermentation
- Citation
- METABOLIC ENGINEERING, v.51, pp.99 - 109
- Indexed
- SCIE
SCOPUS
- Journal Title
- METABOLIC ENGINEERING
- Volume
- 51
- Start Page
- 99
- End Page
- 109
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/68401
- DOI
- 10.1016/j.ymben.2018.08.007
- ISSN
- 1096-7176
- Abstract
- Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His(6)-tag to improve the production of glutaric acid. It was found that use of N-terminal His(6)-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His(6)-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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