Asiatic acid induces apoptosis and autophagy and reduces MiR-17 and MiR-21 expression in pancreatic cancer cell lines
DC Field | Value | Language |
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dc.contributor.author | Jo, Y.-G. | - |
dc.contributor.author | Kim, M. | - |
dc.contributor.author | Shin, H. | - |
dc.contributor.author | Lee, K.Y. | - |
dc.contributor.author | Lee, E.J. | - |
dc.date.accessioned | 2021-09-02T01:19:54Z | - |
dc.date.available | 2021-09-02T01:19:54Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1226-3907 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/70769 | - |
dc.description.abstract | − This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer. © 2019, Korean Society of Pharmacognosy. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Korean Society of Pharmacognosy | - |
dc.subject | asiatic acid | - |
dc.subject | hydroxymethylglutaryl coenzyme A reductase kinase | - |
dc.subject | lipocortin 5 | - |
dc.subject | mammalian target of rapamycin | - |
dc.subject | microRNA | - |
dc.subject | microrna 17 | - |
dc.subject | microRNA 21 | - |
dc.subject | unclassified drug | - |
dc.subject | apoptosis | - |
dc.subject | Article | - |
dc.subject | autophagy (cellular) | - |
dc.subject | cell viability | - |
dc.subject | concentration response | - |
dc.subject | controlled study | - |
dc.subject | cytotoxicity | - |
dc.subject | drug mechanism | - |
dc.subject | drug potency | - |
dc.subject | enzyme activation | - |
dc.subject | human | - |
dc.subject | human cell | - |
dc.subject | MIA PaCa-2 cell line | - |
dc.subject | oncogene | - |
dc.subject | PANC-1 cell line | - |
dc.subject | pancreas cancer | - |
dc.subject | TUNEL assay | - |
dc.subject | Western blotting | - |
dc.title | Asiatic acid induces apoptosis and autophagy and reduces MiR-17 and MiR-21 expression in pancreatic cancer cell lines | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, K.Y. | - |
dc.identifier.doi | 10.20307/nps.2019.25.4.298 | - |
dc.identifier.scopusid | 2-s2.0-85078781581 | - |
dc.identifier.bibliographicCitation | Natural Product Sciences, v.25, no.4, pp.298 - 303 | - |
dc.relation.isPartOf | Natural Product Sciences | - |
dc.citation.title | Natural Product Sciences | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 298 | - |
dc.citation.endPage | 303 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002538381 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | asiatic acid | - |
dc.subject.keywordPlus | hydroxymethylglutaryl coenzyme A reductase kinase | - |
dc.subject.keywordPlus | lipocortin 5 | - |
dc.subject.keywordPlus | mammalian target of rapamycin | - |
dc.subject.keywordPlus | microRNA | - |
dc.subject.keywordPlus | microrna 17 | - |
dc.subject.keywordPlus | microRNA 21 | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | autophagy (cellular) | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | concentration response | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cytotoxicity | - |
dc.subject.keywordPlus | drug mechanism | - |
dc.subject.keywordPlus | drug potency | - |
dc.subject.keywordPlus | enzyme activation | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | MIA PaCa-2 cell line | - |
dc.subject.keywordPlus | oncogene | - |
dc.subject.keywordPlus | PANC-1 cell line | - |
dc.subject.keywordPlus | pancreas cancer | - |
dc.subject.keywordPlus | TUNEL assay | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Asiatic acid | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | MicroRNA | - |
dc.subject.keywordAuthor | Pancreatic cancer | - |
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