Asiatic acid induces apoptosis and autophagy and reduces MiR-17 and MiR-21 expression in pancreatic cancer cell lines
- Authors
- Jo, Y.-G.; Kim, M.; Shin, H.; Lee, K.Y.; Lee, E.J.
- Issue Date
- 2019
- Publisher
- Korean Society of Pharmacognosy
- Keywords
- Apoptosis; Asiatic acid; Autophagy; MicroRNA; Pancreatic cancer
- Citation
- Natural Product Sciences, v.25, no.4, pp.298 - 303
- Indexed
- SCOPUS
KCI
- Journal Title
- Natural Product Sciences
- Volume
- 25
- Number
- 4
- Start Page
- 298
- End Page
- 303
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/70769
- DOI
- 10.20307/nps.2019.25.4.298
- ISSN
- 1226-3907
- Abstract
- − This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer. © 2019, Korean Society of Pharmacognosy. All rights reserved.
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