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Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive

Authors
Joo, Young-ChulKo, Young JinYou, Seung KyouShin, Sang KyuHyeon, Jeong EunMusaad, Almisned ShuaaHan, Sung Ok
Issue Date
26-12월-2018
Publisher
AMER CHEMICAL SOC
Keywords
taurine production; new pathway; metabolic engineering; Corynebacterium glutamicum; food additive
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.51, pp.13454 - 13463
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume
66
Number
51
Start Page
13454
End Page
13463
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/70865
DOI
10.1021/acs.jafc.8b05093
ISSN
0021-8561
Abstract
Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and L-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.
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