Red ginseng-derived saponin fraction suppresses the obesity-induced inflammatory responses via Nrf2-HO-1 pathway in adipocyte-macrophage co-culture system
DC Field | Value | Language |
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dc.contributor.author | Kim, Chae Young | - |
dc.contributor.author | Kang, Bobin | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.contributor.author | Choi, Hyeon-Son | - |
dc.date.accessioned | 2021-09-02T02:39:31Z | - |
dc.date.available | 2021-09-02T02:39:31Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 0753-3322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71451 | - |
dc.description.abstract | The aim of this study was to investigate the effect of saponin fraction (SF) from red ginseng on obesity-induced inflammatory response in a co-culture system of 3T3-L1 and RAW264.7 cells. HPLC analysis showed that SF contains more than 50% ginsenosides, and Rb1 was the most abundant ginsenoside [135.31 mu g/mg (extract)]. The production of nitric oxide and cytokines, induced by adipocyte-conditioned medium (3T3-CM), was significantly decreased by SF. SF (100 mu g/mL) suppressed the abundance of tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), and interleukin-6 (IL-6) by 78%, 40%, and 22%, respectively. This SF-mediated reduction in inflammatory cytokines was due to the suppression of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (I kappa B alpha) phosphorylation, and translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) into the nucleus. SF also regulated adipokine expression in adipocytes, which were stimulated by macrophage-conditioned medium (RAW-CM); adiponectin expression was upregulated (> 2-fold), while resistin was downregulated (40%). In the contact system of adipocytes and macrophages, SF significantly decreases MCP-1 (37%) and IL-6 (25%) production. In the transwell system, SF (100 mu g/mL) significantly increased the abundance of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1) by 1.5 similar to 3.5-fold and 2.8 similar to 3.6-fold, respectively, thus increasing Nrf2 translocation into nucleus. However, SF-mediated inhibitory effect on the release of IL-6 and MCP-1 cytokines was reversed in the Nrf2 or HO-1 knockdown condition. This result indicated that SF-mediated inhibition of obesity-induced inflammation was dependent on Nrf2 activation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | - |
dc.subject | NRF2 SIGNALING PATHWAY | - |
dc.subject | HEME OXYGENASE-1 | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | HIGH-FAT | - |
dc.subject | ACTIVATION | - |
dc.subject | PHARMACOLOGY | - |
dc.subject | ADIPONECTIN | - |
dc.subject | INHIBITION | - |
dc.subject | ROLES | - |
dc.subject | MODEL | - |
dc.title | Red ginseng-derived saponin fraction suppresses the obesity-induced inflammatory responses via Nrf2-HO-1 pathway in adipocyte-macrophage co-culture system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1016/j.biopha.2018.09.169 | - |
dc.identifier.scopusid | 2-s2.0-85054438146 | - |
dc.identifier.wosid | 000450101800167 | - |
dc.identifier.bibliographicCitation | BIOMEDICINE & PHARMACOTHERAPY, v.108, pp.1507 - 1516 | - |
dc.relation.isPartOf | BIOMEDICINE & PHARMACOTHERAPY | - |
dc.citation.title | BIOMEDICINE & PHARMACOTHERAPY | - |
dc.citation.volume | 108 | - |
dc.citation.startPage | 1507 | - |
dc.citation.endPage | 1516 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | NRF2 SIGNALING PATHWAY | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | HIGH-FAT | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PHARMACOLOGY | - |
dc.subject.keywordPlus | ADIPONECTIN | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Obesity-induced inflammation | - |
dc.subject.keywordAuthor | Co-culture | - |
dc.subject.keywordAuthor | Saponin fraction | - |
dc.subject.keywordAuthor | Nuclear factor (erythroid-derived 2)-like 2 | - |
dc.subject.keywordAuthor | (Nrf2)-hemoxygenase-1 (HO-1) signaling pathway | - |
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