Reflection phase microscopy using spatio-temporal coherence of light
- Authors
- Choi, Youngwoon; Hosseini, Poorya; Kang, Jeon Woong; Kang, Sungsam; Yang, Taeseok Daniel; Hyeon, Min Gyu; Kim, Beop-Min; So, Peter T. C.; Yaqoob, Zahid
- Issue Date
- 20-11월-2018
- Publisher
- OPTICAL SOC AMER
- Citation
- OPTICA, v.5, no.11, pp.1468 - 1473
- Indexed
- SCIE
SCOPUS
- Journal Title
- OPTICA
- Volume
- 5
- Number
- 11
- Start Page
- 1468
- End Page
- 1473
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/71822
- DOI
- 10.1364/OPTICA.5.001468
- ISSN
- 2334-2536
- Abstract
- Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase micro- scope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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Collections - Graduate School > Department of Bioengineering > 1. Journal Articles
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