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Graphene-based enzyme-modified field-effect transistor biosensor for monitoring drug effects in Alzheimer's disease treatment

Authors
Chae, Myung-SicYoo, Yong KyoungKim, JinsikKim, Tae GeunHwang, Kyo Seon
Issue Date
1-11월-2018
Publisher
ELSEVIER SCIENCE SA
Keywords
Reduced graphene oxide; Alzheimer' s disease; Biosensor; Acetylcholine; Acetylcholinesterase inhibitor
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.272, pp.448 - 458
Indexed
SCIE
SCOPUS
Journal Title
SENSORS AND ACTUATORS B-CHEMICAL
Volume
272
Start Page
448
End Page
458
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/71897
DOI
10.1016/j.snb.2018.06.010
ISSN
0925-4005
Abstract
A reduced graphene oxide-based enzyme-modified field-effect transistor (RGO-EnFET) was fabricated to study the enzymatic kinetics between acetylcholinesterase (AChE) and acetylcholine (ACh), which are related to the progression and treatment of Alzheimer's disease (AD). The RGO-EnFET exhibited typical ambipolar transfer characteristics with a charge neutrality point (the Dirac point, VDirac) in an aqueous environment, and biological events occurring on the RGO surface were evaluated by observing the Dirac point shift (.VDirac) according to reactions. First, the pH sensitivity of the RGO-EnFET was measured to be 24.12 mV pH(-1) for a pH range of 4-10 with excellent repeatability. Then, ACh quantification in the concentration range of 1 mu M-10mM was evaluated, with a linear slope of 13.9 mV dec(-1) being obtained between.VDirac and the ACh concentration on a logarithmic scale. The effects of AChE inhibitors (donepezil and rivastigmine) on the AChE enzymatic activity were also investigated. The distinguishable inhibition rates were acquired for donepezil and rivastigmine at saturation levels of 90% and 75%, respectively. These results indicate that the RGO-EnFET biosensor was successfully exploited as an in vitro analytical tool and has potential of future application in the study of enzymatic kinetics and drug screening for therapeutic purposes.
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공과대학 (전기전자공학부)
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