reparation of High Purity Delta 5-Olefinic Acids from Pine Nut Oil via Repeated Lipase-Catalyzed Esterification
DC Field | Value | Language |
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dc.contributor.author | Kim, Heejin | - |
dc.contributor.author | Choi, Nakyung | - |
dc.contributor.author | Kim, Hak-Ryul | - |
dc.contributor.author | Lee, Junsoo | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.date.accessioned | 2021-09-02T04:09:02Z | - |
dc.date.available | 2021-09-02T04:09:02Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 1345-8957 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71932 | - |
dc.description.abstract | Delta 5-Olefinic acids have been characterized in gymnosperm plants and have been reported to have several biological health benefits. Delta 5-Olefinic acids from pine nut oil were effectively concentrated by repeated lipase-catalyzed esterification. The pine nut oil contained three major Delta 5-olefinic acids, namely taxoleic acid (C18:2 Delta 5,9), pinolenic acid (C18:3 Delta 5,9,12), and sciadonic acid (C20:3 Delta 5,11,14). The fatty acids present in pine nut oil were selectively esterified with ethanol using Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. The Delta 5-olefinic acids were concentrated in the unesterified fatty acid fraction. The optimum molar ratio of the substrates (fatty acid:ethanol), temperature, the enzyme loading, and the reaction time were 1:7, 25 degrees C, 5% of total substrate weight, and 6 h, respectively. There was no significant effect in the concentration of Delta 5-olefinic acids when water was added in the reaction mixture. The same protocol and optimum conditions were employed for two times repeated lipase-catalyzed esterifications. In first lipase-catalyzed esterification, the Delta 5-olefinic acids content in the pine nut oil increased from 17 mol% to 51 mol% with a yield of 40 mol%. In a second lipase-catalyzed esterification, with the Delta 5-olefinic acids-concentrated fatty acids obtained from the first reaction as the substrate, the Delta 5-olefinic acids content increased to 86 mol% with a yield of 15 mol%. Finally, a maximum Delta 5-olefinic acids content of ca. 96 mol% with a yield of 6 mol% was obtained via a third lipase-catalyzed esterification. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | JAPAN OIL CHEMISTS SOC | - |
dc.subject | PACKED-BED REACTOR | - |
dc.subject | SEED OIL | - |
dc.subject | PINOLENIC ACID | - |
dc.subject | ENZYMATIC ESTERIFICATION | - |
dc.subject | DOCOSAHEXAENOIC ACID | - |
dc.subject | LIPID-METABOLISM | - |
dc.subject | FISH-OIL | - |
dc.subject | ENRICHMENT | - |
dc.subject | SEPARATION | - |
dc.subject | WATER | - |
dc.title | reparation of High Purity Delta 5-Olefinic Acids from Pine Nut Oil via Repeated Lipase-Catalyzed Esterification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.5650/jos.ess18136 | - |
dc.identifier.scopusid | 2-s2.0-85056381190 | - |
dc.identifier.wosid | 000452490600009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF OLEO SCIENCE, v.67, no.11, pp.1435 - 1442 | - |
dc.relation.isPartOf | JOURNAL OF OLEO SCIENCE | - |
dc.citation.title | JOURNAL OF OLEO SCIENCE | - |
dc.citation.volume | 67 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1435 | - |
dc.citation.endPage | 1442 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | PACKED-BED REACTOR | - |
dc.subject.keywordPlus | SEED OIL | - |
dc.subject.keywordPlus | PINOLENIC ACID | - |
dc.subject.keywordPlus | ENZYMATIC ESTERIFICATION | - |
dc.subject.keywordPlus | DOCOSAHEXAENOIC ACID | - |
dc.subject.keywordPlus | LIPID-METABOLISM | - |
dc.subject.keywordPlus | FISH-OIL | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordAuthor | concentration | - |
dc.subject.keywordAuthor | lipase-catalyzed esterification | - |
dc.subject.keywordAuthor | Lipozyme RM IM | - |
dc.subject.keywordAuthor | Delta 5-olefinic acids | - |
dc.subject.keywordAuthor | pine nut oil | - |
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