Design of a 3D BMP-2-Delivering Tannylated PCL Scaffold and Its Anti-Oxidant, Anti-Inflammatory, and Osteogenic Effects In Vitro
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae Yong | - |
dc.contributor.author | Lim, Hyunwoong | - |
dc.contributor.author | Ahn, Jae Won | - |
dc.contributor.author | Jang, Dongsik | - |
dc.contributor.author | Lee, Seung Hee | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Kim, Sung Eun | - |
dc.date.accessioned | 2021-09-02T04:09:22Z | - |
dc.date.available | 2021-09-02T04:09:22Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71935 | - |
dc.description.abstract | In this study, a novel three-dimensional (3D) bone morphogenic protein-2 (BMP-2)-delivering tannylated polycaprolactone (PCL) (BMP-2/tannic acid (TA)/PCL) scaffold with anti-oxidant, anti-inflammatory, and osteogenic activities was fabricated via simple surface coating with TA, followed by the immobilization of BMP-2 on the TA-coated PCL scaffold. The BMP-2/TA/PCL scaffold showed controlled and sustained BMP-2 release. It effectively scavenged reactive oxygen species (ROS) in cells, and increased the proliferation of MC3T3-E1 cells pre-treated with hydrogen peroxide (H2O2). Additionally, the BMP-2/TA/PCL scaffold significantly suppressed the mRNA levels of pro-inflammatory cytokines, including matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor- (TNF-), in lipopolysaccharide (LPS)-induced MC3T3-E1 cells. Furthermore, it showed outstanding enhancement of the osteogenic activity of MC3T3-E1 cells through increased alkaline phosphatase (ALP) activity and calcium deposition. Our findings demonstrated that the BMP-2/TA/PCL scaffold plays an important role in scavenging ROS, suppressing inflammatory response, and enhancing the osteogenic differentiation of cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | TANNIC-ACID | - |
dc.subject | MORPHOGENETIC PROTEINS | - |
dc.subject | CELLS | - |
dc.subject | TITANIUM | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | GELATIN | - |
dc.subject | RELEASE | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | NANOPARTICLES | - |
dc.title | Design of a 3D BMP-2-Delivering Tannylated PCL Scaffold and Its Anti-Oxidant, Anti-Inflammatory, and Osteogenic Effects In Vitro | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jae Yong | - |
dc.contributor.affiliatedAuthor | Jang, Dongsik | - |
dc.contributor.affiliatedAuthor | Kim, Sung Eun | - |
dc.identifier.doi | 10.3390/ijms19113602 | - |
dc.identifier.scopusid | 2-s2.0-85056698569 | - |
dc.identifier.wosid | 000451528500318 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.11 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 19 | - |
dc.citation.number | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | TANNIC-ACID | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEINS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TITANIUM | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | GELATIN | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordAuthor | bone morphogenic protein-2 (BMP-2) | - |
dc.subject.keywordAuthor | tannic acid (TA) | - |
dc.subject.keywordAuthor | polycaprolactone (PCL) | - |
dc.subject.keywordAuthor | 3D scaffold | - |
dc.subject.keywordAuthor | anti-oxidation | - |
dc.subject.keywordAuthor | anti-inflammation | - |
dc.subject.keywordAuthor | osteogenic differentiation | - |
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