Design of a 3D BMP-2-Delivering Tannylated PCL Scaffold and Its Anti-Oxidant, Anti-Inflammatory, and Osteogenic Effects In Vitro
- Authors
- Lee, Jae Yong; Lim, Hyunwoong; Ahn, Jae Won; Jang, Dongsik; Lee, Seung Hee; Park, Kyeongsoon; Kim, Sung Eun
- Issue Date
- 11월-2018
- Publisher
- MDPI
- Keywords
- bone morphogenic protein-2 (BMP-2); tannic acid (TA); polycaprolactone (PCL); 3D scaffold; anti-oxidation; anti-inflammation; osteogenic differentiation
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.11
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 19
- Number
- 11
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/71935
- DOI
- 10.3390/ijms19113602
- ISSN
- 1661-6596
- Abstract
- In this study, a novel three-dimensional (3D) bone morphogenic protein-2 (BMP-2)-delivering tannylated polycaprolactone (PCL) (BMP-2/tannic acid (TA)/PCL) scaffold with anti-oxidant, anti-inflammatory, and osteogenic activities was fabricated via simple surface coating with TA, followed by the immobilization of BMP-2 on the TA-coated PCL scaffold. The BMP-2/TA/PCL scaffold showed controlled and sustained BMP-2 release. It effectively scavenged reactive oxygen species (ROS) in cells, and increased the proliferation of MC3T3-E1 cells pre-treated with hydrogen peroxide (H2O2). Additionally, the BMP-2/TA/PCL scaffold significantly suppressed the mRNA levels of pro-inflammatory cytokines, including matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor- (TNF-), in lipopolysaccharide (LPS)-induced MC3T3-E1 cells. Furthermore, it showed outstanding enhancement of the osteogenic activity of MC3T3-E1 cells through increased alkaline phosphatase (ALP) activity and calcium deposition. Our findings demonstrated that the BMP-2/TA/PCL scaffold plays an important role in scavenging ROS, suppressing inflammatory response, and enhancing the osteogenic differentiation of cells.
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