Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction
- Authors
- Hong, Min Eui; Choi, Hong Il; Kwak, Ho Seok; Hwang, Sung-Won; Sung, Young Joon; Chang, Won Seok; Sim, Sang Jun
- Issue Date
- 11월-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Haematococcus pluvialis; Autotrophic astaxanthin production; High-throughput screening; Azide-based colorimetric assay; Oil-based astaxanthin extraction method
- Citation
- BIORESOURCE TECHNOLOGY, v.267, pp.175 - 181
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 267
- Start Page
- 175
- End Page
- 181
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/72030
- DOI
- 10.1016/j.biortech.2018.07.033
- ISSN
- 0960-8524
- Abstract
- The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 mu M of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 +/- 5.69 mg L-1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating micro-algae with high astaxanthin production.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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