Methylobacterium currus sp nov., isolated from a car air conditioning system
- Authors
- Park, Chulwoo; Lee, Yun Suk; Park, So-yoon; Park, Woojun
- Issue Date
- 11월-2018
- Publisher
- MICROBIOLOGY SOC
- Keywords
- Methylobacterium; Alphaproteobacteria; taxonomy
- Citation
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v.68, no.11, pp.3621 - 3626
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Volume
- 68
- Number
- 11
- Start Page
- 3621
- End Page
- 3626
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/72081
- DOI
- 10.1099/ijsem.0.003045
- ISSN
- 1466-5026
- Abstract
- A novel bacterial strain, designated PR1016A(T), was isolated from a car air conditioning system. This rod-shaped strain showed catalase and oxidase activities, was aerobic and methylotrophic, and had a reddish pink colour. The genomic DNA G+C content of strain PR1016A(T) was 70.2 mol%, as determined by genome sequencing. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain PR1016A(T) was most closely related to Methylobacterium aquaticum GR16(T) (98.86 %), M. variabile GR3(T) (98.43 %), M. platani PMB 02(T) (98.36 %) and M. tarhaniae N4211(T) (98.14 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain PR1016A(T) and M. aquaticum GR16(T), M. platani PMB02 T and M. variabile GR3(T) were 88.61, 88.14 and 87.88 %, and 36.4, 35.8 and 34.7 %, respectively. Numerous insertion sequences are present in the genome of strain PR1016A T, which has a larger genome than the four Methylobacterium species described above. Cells grew at 18-42 degrees C (optimum, 30 ffi C), at pH 4.0-9.0 (optimum, pH 7.0) and in the presence of 0-1.0% (w/v) NaCl (optimum, 0 %). The major respiratory quinone was Q10. Fatty acid methyl ester analysis revealed that summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) was the predominant cellular fatty acid in strain PR1016A(T). Two-dimensional TLC indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The genotypic and phenotypic characteristics indicate that strain PR1016A(T) represents a novel species of the genus Methylobacterium, for which the name Methylobacterium currus sp. nov. is proposed. The type strain is PR1016A(T) (= KACC 19662(T) = JCM 32670(T)).
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Collections - College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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