Electropreconcentration, gate injection, and capillary electrophoresis separation on a microchip
DC Field | Value | Language |
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dc.contributor.author | Chun, Honggu | - |
dc.date.accessioned | 2021-09-02T05:04:44Z | - |
dc.date.available | 2021-09-02T05:04:44Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-10-19 | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/72462 | - |
dc.description.abstract | The nanochannel-based electropreconcentration is not compatible with successive capillary zone electrophoresis (CZE). In this study, the incompatibility is theoretically discussed and experimentally proven, and then, the development of a monolithic glass microfluidic chip for performing integrated electropreconcentration and CZE separation is described. The sample is electropreconcentrated at the interface of a micro- and nanochannel where electric double layer overlap conditions exist. Because an ion-depletion region develops at the leading front of the preconcentrated plug, a field-enhanced sample stacking effect occurs which limits the separation efficiency unless compensated for. The ion-depletion region was confirmed by monitoring the solution conductivity at discrete points in the microchannel during the preconcentration step. The solution conductivity decreased >20-fold during the preconcentration step. To overcome the effects of this region, a cross-intersection was used to shunt the ion-depleted buffer away from the analysis channel while reintroducing the running buffer. When the preconcentrated sample plug arrives at the cross-intersection, it is gate injected into the analysis channel so that fresh running buffer surrounds the plug. Under these conditions, three-peptide mixture was preconcentrated 200 fold in 60 s and the preconcentrated plug was successfully resolved with better than 1% relative standard deviations in migration times. (C) 2018 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | ION CONCENTRATION POLARIZATION | - |
dc.subject | SAMPLE PRECONCENTRATION | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | ELECTROSPRAY-IONIZATION | - |
dc.subject | STACKING | - |
dc.subject | CHARGE | - |
dc.subject | CHIP | - |
dc.subject | PROTEINS | - |
dc.subject | DEPLETION | - |
dc.subject | MEMBRANE | - |
dc.title | Electropreconcentration, gate injection, and capillary electrophoresis separation on a microchip | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1016/j.chroma.2018.08.053 | - |
dc.identifier.scopusid | 2-s2.0-85053074837 | - |
dc.identifier.wosid | 000446287000022 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHY A, v.1572, pp.179 - 186 | - |
dc.relation.isPartOf | JOURNAL OF CHROMATOGRAPHY A | - |
dc.citation.title | JOURNAL OF CHROMATOGRAPHY A | - |
dc.citation.volume | 1572 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 186 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | ION CONCENTRATION POLARIZATION | - |
dc.subject.keywordPlus | SAMPLE PRECONCENTRATION | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | ELECTROSPRAY-IONIZATION | - |
dc.subject.keywordPlus | STACKING | - |
dc.subject.keywordPlus | CHARGE | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | DEPLETION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordAuthor | Electropreconcentration | - |
dc.subject.keywordAuthor | Ion concentration polarization | - |
dc.subject.keywordAuthor | Capillary electrophoresis | - |
dc.subject.keywordAuthor | Gate injection | - |
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