Electropreconcentration, gate injection, and capillary electrophoresis separation on a microchip
- Authors
- Chun, Honggu
- Issue Date
- 19-10월-2018
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Electropreconcentration; Ion concentration polarization; Capillary electrophoresis; Gate injection
- Citation
- JOURNAL OF CHROMATOGRAPHY A, v.1572, pp.179 - 186
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHROMATOGRAPHY A
- Volume
- 1572
- Start Page
- 179
- End Page
- 186
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/72462
- DOI
- 10.1016/j.chroma.2018.08.053
- ISSN
- 0021-9673
- Abstract
- The nanochannel-based electropreconcentration is not compatible with successive capillary zone electrophoresis (CZE). In this study, the incompatibility is theoretically discussed and experimentally proven, and then, the development of a monolithic glass microfluidic chip for performing integrated electropreconcentration and CZE separation is described. The sample is electropreconcentrated at the interface of a micro- and nanochannel where electric double layer overlap conditions exist. Because an ion-depletion region develops at the leading front of the preconcentrated plug, a field-enhanced sample stacking effect occurs which limits the separation efficiency unless compensated for. The ion-depletion region was confirmed by monitoring the solution conductivity at discrete points in the microchannel during the preconcentration step. The solution conductivity decreased >20-fold during the preconcentration step. To overcome the effects of this region, a cross-intersection was used to shunt the ion-depleted buffer away from the analysis channel while reintroducing the running buffer. When the preconcentrated sample plug arrives at the cross-intersection, it is gate injected into the analysis channel so that fresh running buffer surrounds the plug. Under these conditions, three-peptide mixture was preconcentrated 200 fold in 60 s and the preconcentrated plug was successfully resolved with better than 1% relative standard deviations in migration times. (C) 2018 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Bioengineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.