Use of DNA aptamer for sandwich type detection of Listeria monocytogenes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Suh, Soo Hwan | - |
dc.contributor.author | Choi, Soo Jung | - |
dc.contributor.author | Dwivedi, Hari P. | - |
dc.contributor.author | Moore, Matthew D. | - |
dc.contributor.author | Escudero-Abarca, Blanca, I | - |
dc.contributor.author | Jaykus, Lee-Ann | - |
dc.date.accessioned | 2021-09-02T06:24:42Z | - |
dc.date.available | 2021-09-02T06:24:42Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-09-15 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/73089 | - |
dc.description.abstract | A single stranded (ss) DNA aptamer, specific to members of Listeria genus, was used to develop a two-site binding sandwich assay for capture and detection of L. monocytogenes. Antibody-immobilized immunomagnetic beads were used to capture L. monocytogenes, followed by their exposure to the aptamer detector. Detection was achieved by amplification of cell-bound aptamers by qPCR. The lower limit of detection for the combined assay was 2.5 CFU L. monocytogenes in 500 mu l buffer. This is juxtaposed to a detection limit of 2.4 log(10) CFU in 500 mu l buffer for immunomagnetic separation coupled with qPCR detection of L monocytogenes targeting the lily gene. When applied to turkey deli meat, subjected to 24 h of non-selective enrichment, the two-site binding sandwich assay showed positive results at initial inoculum levels of 1-2 log(10) CFU per 25 g sample. Because of its lower limit of detection, the assay reported here could be useful for detection of L monocytogenes in foods and environmental samples. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | ESCHERICHIA-COLI O157-H7 | - |
dc.subject | IMMUNOMAGNETIC SEPARATION | - |
dc.subject | NONSPECIFIC-BINDING | - |
dc.subject | SELECTION | - |
dc.subject | CAPTURE | - |
dc.subject | ASSAY | - |
dc.subject | CONJUNCTION | - |
dc.subject | PROTEINS | - |
dc.subject | ELISA | - |
dc.title | Use of DNA aptamer for sandwich type detection of Listeria monocytogenes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Soo Jung | - |
dc.identifier.doi | 10.1016/j.ab.2018.04.009 | - |
dc.identifier.scopusid | 2-s2.0-85049935718 | - |
dc.identifier.wosid | 000442711700005 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.557, pp.27 - 33 | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 557 | - |
dc.citation.startPage | 27 | - |
dc.citation.endPage | 33 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157-H7 | - |
dc.subject.keywordPlus | IMMUNOMAGNETIC SEPARATION | - |
dc.subject.keywordPlus | NONSPECIFIC-BINDING | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | CAPTURE | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | CONJUNCTION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ELISA | - |
dc.subject.keywordAuthor | Listeria monocytogenes | - |
dc.subject.keywordAuthor | Magnetic bead | - |
dc.subject.keywordAuthor | Nucleic acid aptamer | - |
dc.subject.keywordAuthor | Pathogen detection | - |
dc.subject.keywordAuthor | Two-site binding sandwich assay | - |
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