Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Use of DNA aptamer for sandwich type detection of Listeria monocytogenes

Authors
Suh, Soo HwanChoi, Soo JungDwivedi, Hari P.Moore, Matthew D.Escudero-Abarca, Blanca, IJaykus, Lee-Ann
Issue Date
15-9월-2018
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
Listeria monocytogenes; Magnetic bead; Nucleic acid aptamer; Pathogen detection; Two-site binding sandwich assay
Citation
ANALYTICAL BIOCHEMISTRY, v.557, pp.27 - 33
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL BIOCHEMISTRY
Volume
557
Start Page
27
End Page
33
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/73089
DOI
10.1016/j.ab.2018.04.009
ISSN
0003-2697
Abstract
A single stranded (ss) DNA aptamer, specific to members of Listeria genus, was used to develop a two-site binding sandwich assay for capture and detection of L. monocytogenes. Antibody-immobilized immunomagnetic beads were used to capture L. monocytogenes, followed by their exposure to the aptamer detector. Detection was achieved by amplification of cell-bound aptamers by qPCR. The lower limit of detection for the combined assay was 2.5 CFU L. monocytogenes in 500 mu l buffer. This is juxtaposed to a detection limit of 2.4 log(10) CFU in 500 mu l buffer for immunomagnetic separation coupled with qPCR detection of L monocytogenes targeting the lily gene. When applied to turkey deli meat, subjected to 24 h of non-selective enrichment, the two-site binding sandwich assay showed positive results at initial inoculum levels of 1-2 log(10) CFU per 25 g sample. Because of its lower limit of detection, the assay reported here could be useful for detection of L monocytogenes in foods and environmental samples.
Files in This Item
There are no files associated with this item.
Appears in
Collections
ETC > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE