A copper transcription factor, AfMac1 regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Yong-Sung | - |
dc.contributor.author | Kang, Suzie | - |
dc.contributor.author | Seo, Hyewon | - |
dc.contributor.author | Yun, Cheol-Won | - |
dc.date.accessioned | 2021-09-02T06:26:52Z | - |
dc.date.available | 2021-09-02T06:26:52Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-09-14 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/73107 | - |
dc.description.abstract | Although iron and copper are co-ordinately regulated in living cells, the homeostatic effects of each of these metals on the other remain unknown. Here, we show the function of AfMac1, a transcriptional activator of the copper and iron regulons of Aspergillus fumigatus, on the interaction between iron and copper. In addition to the copper-specific AfMac1-binding motif 5'-TGTGCTCA-3' found in the promoter region of ctrC, the iron specific AfMac1-binding motif 5'-AT(C/G)NN(A/T)T(A/C)-3' was identified in the iron regulon but not in the copper regulon by ChIP sequence analysis. Furthermore, mutation of the AfMac1-binding motif of sit1 eliminated AfMac1-mediated sit1 up-regulation. Interestingly, the regulation of gene expression in the iron regulon by AfMac1 was not affected by copper and vice versa. AfMac1 localized to the nucleus under iron- or copper-depleted conditions, and AfMac1 was mostly detected in the cytoplasm under iron- or copper-replete conditions. Taken together, these results suggest that A. fumigatus independently regulates iron and copper homeostasis in a manner that involves AfMac1 and mutual interactions. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | CRYPTOCOCCUS-NEOFORMANS | - |
dc.subject | SIDEROPHORE-IRON | - |
dc.subject | YEAST CCC2 | - |
dc.subject | SUBUNIT-II | - |
dc.subject | TRANSPORTER | - |
dc.subject | OXIDASE | - |
dc.subject | DISEASE | - |
dc.subject | BINDING | - |
dc.subject | PROTEIN | - |
dc.title | A copper transcription factor, AfMac1 regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Cheol-Won | - |
dc.identifier.doi | 10.1042/BCJ20180399 | - |
dc.identifier.scopusid | 2-s2.0-85053140135 | - |
dc.identifier.wosid | 000444276800008 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL JOURNAL, v.475, pp.2831 - 2845 | - |
dc.relation.isPartOf | BIOCHEMICAL JOURNAL | - |
dc.citation.title | BIOCHEMICAL JOURNAL | - |
dc.citation.volume | 475 | - |
dc.citation.startPage | 2831 | - |
dc.citation.endPage | 2845 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | CRYPTOCOCCUS-NEOFORMANS | - |
dc.subject.keywordPlus | SIDEROPHORE-IRON | - |
dc.subject.keywordPlus | YEAST CCC2 | - |
dc.subject.keywordPlus | SUBUNIT-II | - |
dc.subject.keywordPlus | TRANSPORTER | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PROTEIN | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.