A copper transcription factor, AfMac1 regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus
- Authors
- Park, Yong-Sung; Kang, Suzie; Seo, Hyewon; Yun, Cheol-Won
- Issue Date
- 14-9월-2018
- Publisher
- PORTLAND PRESS LTD
- Citation
- BIOCHEMICAL JOURNAL, v.475, pp.2831 - 2845
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL JOURNAL
- Volume
- 475
- Start Page
- 2831
- End Page
- 2845
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/73107
- DOI
- 10.1042/BCJ20180399
- ISSN
- 0264-6021
- Abstract
- Although iron and copper are co-ordinately regulated in living cells, the homeostatic effects of each of these metals on the other remain unknown. Here, we show the function of AfMac1, a transcriptional activator of the copper and iron regulons of Aspergillus fumigatus, on the interaction between iron and copper. In addition to the copper-specific AfMac1-binding motif 5'-TGTGCTCA-3' found in the promoter region of ctrC, the iron specific AfMac1-binding motif 5'-AT(C/G)NN(A/T)T(A/C)-3' was identified in the iron regulon but not in the copper regulon by ChIP sequence analysis. Furthermore, mutation of the AfMac1-binding motif of sit1 eliminated AfMac1-mediated sit1 up-regulation. Interestingly, the regulation of gene expression in the iron regulon by AfMac1 was not affected by copper and vice versa. AfMac1 localized to the nucleus under iron- or copper-depleted conditions, and AfMac1 was mostly detected in the cytoplasm under iron- or copper-replete conditions. Taken together, these results suggest that A. fumigatus independently regulates iron and copper homeostasis in a manner that involves AfMac1 and mutual interactions.
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