Microwave-induced formation of oligomeric amyloid aggregates
- Authors
- Lee, Wonseok; Choi, Yeseong; Lee, Sang Won; Kim, Insu; Lee, Dongtak; Hong, Yoochan; Lee, Gyudo; Yoon, Dae Sung
- Issue Date
- 24-8월-2018
- Publisher
- IOP PUBLISHING LTD
- Keywords
- amyloid aggregation; microwave irradiation; oligomeric amyloid aggregate; L-ascorbic acid; silver nanomaterial; drug-screen
- Citation
- NANOTECHNOLOGY, v.29, no.34
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANOTECHNOLOGY
- Volume
- 29
- Number
- 34
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/73732
- DOI
- 10.1088/1361-6528/aac8f4
- ISSN
- 0957-4484
- Abstract
- Amyloid aggregates have emerged as a significant hallmark of neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Although it has been recently reported that microwave heating induces amyloid aggregation compared with conventional heating methods, the mechanism of amyloid aggregate induction has remained unclear. In this study, we investigated the formation of oligomeric amyloid aggregates (OAAs) by microwave irradiation at microscale volumes of solution. Microwave irradiation of protein monomer solution triggered rapid formation of OAAs within 7 min. We characterized the formation of OAAs using atomic force microscopy, thioflavin T fluorescent assay and circular dichroism. In the microwave system, we also investigated the inhibitory effect on the formation of amyloid aggregates by L-ascorbic acid as well as enhanced amyloid aggregation by silver nanomaterials such as nanoparticles and nanowires. We believe that microwave technology has the potential to facilitate the study of amyloid aggregation in the presence of chemical agents or nanomaterials.
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- Appears in
Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
- Graduate School > Department of Bioengineering > 1. Journal Articles
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