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Ecdysone-responsive microRNA-252-5p controls the cell cycle by targeting Abi in Drosophila

Authors
Lim, Do-HwanLee, SeungjaeHan, Jee YunChoi, Min-SeokHong, Jae-SangSeong, YoungmoKwon, Young-SooLee, Young Sik
Issue Date
8월-2018
Publisher
FEDERATION AMER SOC EXP BIOL
Keywords
20E; Ago1; CLIP-seq; microRNA; cyclin
Citation
FASEB JOURNAL, v.32, no.8, pp.4519 - 4533
Indexed
SCIE
SCOPUS
Journal Title
FASEB JOURNAL
Volume
32
Number
8
Start Page
4519
End Page
4533
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/73887
DOI
10.1096/fj.201701185RR
ISSN
0892-6638
Abstract
The steroid hormone ecdysone has a central role in the developmental transitions of insects through its control of responsive protein-coding and microRNA (miRNA) gene expression. However, the complete regulatory network controlling the expression of these genes remains to be elucidated. In this study, we performed cross-linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 (Ago1) protein, the core effector of the miRNA pathway, in Drosophila S2 cells. We found that regulatory interactions between miRNAs and their cognate targets were substantially altered by Ago1 in response to ecdysone signaling. Additionally, during the larva-to-adult metamorphosis, miR-252-5p was up-regulated via the canonical ecdysone-signaling pathway. Moreover, we provide evidence that miR-252-5p targets Abelson interacting protein (Abi) to decrease the protein levels of cyclins A and B, controlling the cell cycle. Overall, our data suggest a potential role for the ecdysone/miR-252-5p/Abi regulatory axis partly in cell-cycle control during metamorphosis in Drosophila.Lim, D.-H., Lee, S., Han, J. Y., Choi, M.-S., Hong, J.-S., Seong, Y., Kwon, Y.-S., Lee, Y. S. Ecdysone-responsive microR-252-5p controls the cell cycle by targeting Abi in Drosophila.
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